The fungal, Yeast rRNA genes consist of the following 18S, ITS1,5.8S, ITS2, 26S, intergenic spacer (IGS) 1, 5S, and IGS2 regions. In addition to ITS, analysis of D1/D2 26S rDNA sequences has more advantage. It enables species identification and also permits phylogenetic analysis. When the DNA sequence data from fungal/yeast isolates have not been registered in DNA data libraries/Gene banks and the isolate cannot be identified to the species level, But with the 26S rDNA D1/D2 sequence genus or family of the isolate can be identified from molecular phylogenetic analyses. It is very difficult to deduce the phylogenetic position of a genus or family of an isolate from the ITS regions as they are more diverse than the 26S region. 26S rDNA D1/D2 sequences of most yeast species have already been determined.
We provide the sequencing of ITS region and 26S rRNA (D1/D2 region) using the ITS1 & LR3R primers.