Random amplified polymorphic DNA (RAPD)

RAPD technique is one type of polymerase chain reaction (PCR) but in this technique segments of DNA  are amplified at random. It is most commonly used molecular techniques in developing the DNA markers. This technique is a modification of the PCR by which a single, short and arbitrary oligonucleotide primer, are able to anneal and prime at multiple locations throughout the genome. It can produce a spectrum of amplified products that are characteristics of the template DNA. RAPD markers have  the wide range of applications in population genetics, gene mapping, molecular evolutionary genetics, animal breeding and plant. This is mainly due to the speed, cost and efficiency of this technique is to generate the large numbers of markers in  the short period compared with other methods. It has the advantage that no prior knowledge of that genome under research is necessary.

Various Application of RAPD:

It has become widely used in the study of

  • Genetic diversity/polymorphism,
  • Germplasm characterization,
  • Genetic structure of populations,
  • Domestication,
  • Detection of somaclonal variation,
  • Cultivar identification,
  • Hybrid purity,
  • Genome mapping,
  • Developing genetic markers linked to a
  • Trait in question,
  • Population and evolutionary genetics,
  • Plant and animal breeding,
  • Animal-plant-microbe interactions
  • Animal behavior study
  • Forensic studies