Reverse transcription-polymerase chain reaction (RT-PCR ) is a sensitive technique for mRNA detection and quantitative analysis. It is one of the best technology to quantify the mRNA level compared with other available technology such us Northern blot analysis and RNase protection assay.
Latest Instrument / platforms enable the detection of PCR products on real-time basis. It has the different wavelength LED to quantify real-time amplification. It is the best method of choice for quantitative analysis for changes in gene expression.
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