HPLC - separation technique; which involves small volume of the liquid sample injection into the stationary phase, in that phase individual components of the sample moved with a liquid mobile phase forced by high pressure. In HPLC, the components are separated from one by one. The column packing interacts between their molecules by various chemical and/or physical interactions with the packing particles. At the exit of this tube column these separated components are detected by the device flow through detector measures their amount. The output from the detector is called a chromatogram.
For the identification of individual compounds in the sample
To measure the amount of a compound in a sample