Next Generation Sequencing

Yaazh Xenomics is one of the leading Next Generation Sequencing service provider in India. Currently we offer Next Generation sequencing services using multiple Next-Generation sequencing platforms including Nanopore, Illumina and Ion Torrent.

Delivering complete range of Next Generation analysis techniques

Whole genome sequencing :

De novo assembly, Scaffolding and finishing, Variant analysis: structural variation, Variant analysis: SNVs, phasing, Resequencing.

Targeted sequencing :

Panels – amplicons, sequence capture, exome, 16S rRNA analysis, 18s rRNA Analysis

Metagenomics :

To analyse the Microbial Diversity in the enviromental samples, water & Soil etc.,

RNA sequencing :

Splice variant analysis, Transcriptome / gene expression, Fusion transcript analysis

Latest 3rd Generation sequencing platforms are offering quality data at affordable pricing for a wide variety of sequencing services.

Nanopore:

The latest Nanotechnology offers Direct DNA/RNA sequencing in Real Time. The Nanopore Next Generation sequencing systems allow you to achieve ultra-long sequencing reads with uniform coverage with no bias across GC content rich regions. This technology is proven with a variety of input material such as genomic DNA, amplified DNA, cDNA and RNA.

The Nanopore is ideal for whole genome de novo assembly projects that require higher-quality data, microbial genome assembly, identifying DNA modifications without bisulfite treatment, and identifying large structural variants. The system delivers up to 99% accuracy.

Learn More about the instrument using below link:

  • https://nanoporetech.com/learn-more
  • Illumina HiSeq / Miseq :

    The Illumina HiSeq / MiSeq systems are most powerful sequencing machines on the market, and it is used by most major genome centers and leading institutions around the world. These machines offer ultra-high-throughput sequencing. Perform production-scale, high-throughput exome, Metagenome and transcriptome sequencing projects quickly and economically.

      Ion Torrent Platform

      The Ion Torrent Personal Genome Machine (PGM) is a small benchtop sequencer. When a nucleotide is incorporated into a strand of DNA by a polymerase, a hydrogen ion (H+) is released as a byproduct. This hydrogen ion carries a charge which the PGM™ System’s ion sensor can detect and convert into a base call.

      Learn More about the instrument using below link:

    • https://www.thermofisher.com/in/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-run-sequence/ion-pgm-system-for-next-generation-sequencing.html

    Next Genration sequencing Principle:

    NGS technology is similar to Capillary Electrophoresis—the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a DNA template strand. NGS extends this process across millions of reactions. The newly identified strings of bases, called reads, are then reassembled using a known reference genome as a scaffold (resequencing), or in the absence of a reference genome (de novo sequencing). The full set of aligned reads reveals the entire sequence of each chromosome in the gDNA sample. This advance enables rapid sequencing of large stretches of DNA base pairs spanning entire genomes. The latest instruments capable of producing hundreds of gigabases of data in a single sequencing run.

    Our services can be divided in the following applications

    • DNA analysis - De novo sequencing, Resequencing/SNP discovery, Structural variation, Whole exome sequencing, Custom targeted sequencing.
    • Transcriptome analysis - Whole transcriptome sequencing, Small RNA analysis
    • Epigenomic analysis - Chip sequencing>

    Whole Genome Sequence : (De novo Sequence & Resequencing)

    It is used to sequence uncharacterized genomes where there is no reference sequence available, or known genomes where significant structural variation is expected.

    Application :

    • Sequencing of unkown genomes or when no reference sequence is available
    • Samples with expected large structural variation e.g. cancer cells
    • Microbial sequencing – experimental strains, genomes with high plasticity

    De novo Sequence Work Flow :

    Whole Genome Sequence

    Whole Exome Sequencing :

    Exome sequencing is an innovative technique, which targets exon region. The exome is defined as the protein coding exons of genes, which make up ≈ 50 MB of the human genome – It is about 1.5% of the entire genome. New sample preparation Technique make it possible to capture this portion of the genome in a single step in a single tube. Exomes are ideal to help us understand high-penetrance allelic variation and its relationship to phenotype.

    Application:

    • Identify most disease-associated variations located with in exons
    • Capture both common and rare variants
    • Sequence about 1% human genome, quickly and highly cost-effective

    Exome Sequence Work Flow:

    Exome Sequence

    Targeted Region Sequencing :

    The advance in technology helps scientists to known more about the genetic bases of many complex diseases utilizing various genetic analysis approaches and methods. Some diseases & disorders have strong associations with certain chromosomes or mutations in certain genes. Based on this knowledge researchers focusing on targeted genomic.

    Work Flow :

    Targeted Region Sequencing

    Features :

    • Focusing on genetic variants/Chromosome of interest
    • Deeper investigation on specific regions/Gene
    • Faster turnaround
    • Higher throughput