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Whole Genome Sequencing

Whole genome sequencing (WGS) or complete genome sequencing or entire genome sequencing or full genome sequencing is that determines the complete DNA sequence of particular organism's genome. WGS produces the comprehensive set of data for an individual organism, and it allows detailed evaluation of all genetic variations on the individual or strain. WGS used as a comprehensive tool for detecting the rare variants and structural variations in the individual's genome study.

Whole Genome : De novo Sequencing

e novo sequencing is typically performed without the aid of a reference genome. It has proven successful for confirming and expanding upon the results from the database searches. It is used as a more comprehensive maps for the genetic variations. We provide various combination of sequencing platforms such us HiSeq, MiSeq, GS-FLX. But the MiSeq platform is the best suited for generating de novo sequence assemblies for smaller genome size organisms. Data used in Gene annotation and prediction.

Whole Genome : Re-sequencing

WGS - Resequencing the reference sequence of a particular genome is available, the sample that can be sequenced will be covered at a lower level and the reads mapped to the reference.

In this we can obtain organism's individual variation from the genome sequence such as single nucleotide polymorphism (SNP), copy number variations (CNV), somatic mutations, and other structural variations. This information are further utilized for discovering certain disease risks, and it plays the role of molecular biomarkers for disease diagnosis and prediction.

Various Next Generation Platforms Available for the Whole Genome Sequence Study:

  • HiSeq2000/2500
  • HiSeq X Ten
  • MiSeq (Paired-end, Mate pair)
  • GS-FLX Titanium/Plus (Shotgun, Mate pair)

Technology Pipline for Whole Genome De novo Sequencing and Re sequencing:

NGS1

 

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