Primer walking service

Analyzing the base sequences of PCR products or plasmid, which is not analyzable by single primer extension in one time. Internal primers are designed and prepared with results obtained after performing end sequencing using the primers given or designated by customers. We make extension continuously by designing an internal primer at an appropriate point using he result obtained from the reaction conducted with a new primer and the same template

This service is to read 2~15kb of the sequence with PCR product or plasmid DNA that cannot be read fully by single primer extension sequencing. One week is taken to read 1~1.5kb by primer walking.