Sanger Sequencing

Sanger Sequencing

DNA Services

Introduction:

Sanger based DNA sequencing is the process of selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. Sanger’s DNA sequencing method is referred as chain termination dideoxy sequencing. This sequencing process use dideoxynucleotides (ddNTP’s) in addition to the normal nucleotides (NTP’s) found in DNA. Dideoxynucleotides are same as nucleotides except they contain a hydrogen group on the 3’ carbon instead of a hydroxyl group (OH). These modified nucleotides, when integrated into a sequence, prevent the addition of further nucleotides. This occurs because a phosphodiester bond cannot form between the dideoxynucleotide and the next incoming nucleotide, and thus the DNA chain is terminated.

Advantage of Yaazh Xenomics Sanger Sequencing Service:

Yaazh Xenomics Laboratory provide High Quality - DNA sequencing read lengths up to ~1000 bases per reaction. A typical read will provide 800 bases with QV20.

Our advanced proprietary optimized technologies allow for consistent, reliable data even on the most difficult templates including hairpins, uneven base distributions and GC-rich region. Free Vector Primers and wide range of Universal Primers are available.

We provide other related cost-effective DNA analysis services at our laboratory including DNA fragment analysis, genotyping and more.

Fast turnaround time

Quality assurance

  • Sample DNA normalisation
  • PCR clean-up
  • Multi-step quality control procedures

Our Lab accept the Wide spectrum of DNA samples

  • Plasmids
  • Cosmids
  • Purified PCR products
  • Un Purified PCR products
  • Phage DNA (single-stranded)
  • Genomic DNA

Dedicated R&D and tech support teams for Troubleshooting, NCBI Submission and other technical inquiries.

 

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