Sequencing

26s rRNA Sequencing

DNA Services

The fungal, Yeast rRNA genes consist of the following 18S, ITS1,5.8S, ITS2, 26S, intergenic spacer (IGS) 1, 5S, and IGS2 regions. In addition to ITS, analysis of D1/D2 26S rDNA sequences has more advantage. It enables species identification and also permits phylogenetic analysis. When the DNA sequence data from fungal/yeast isolates have not been registered in DNA data libraries/Gene banks and the isolate cannot be identified to the species level, But with the 26S rDNA D1/D2 sequence genus or family of the isolate can be identified from molecular phylogenetic analyses. It is very difficult to deduce the phylogenetic position of a genus or family of an isolate from the ITS regions as they are more diverse than the 26S region. 26S rDNA D1/D2 sequences of most yeast species have already been determined.

We provide the sequencing of ITS region and 26S rRNA (D1/D2 region) using the ITS1 & LR3R primers.

 

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