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Restriction Fragment Length Polymorphism (RFLP)

RFLP is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the patterns generated can be used to differentiate species (and even strains) from one another. Polymorphisms are inherited differences found among the individuals in more than 1% of normal population.

Applications of RFLP

RFLPs can be used in many different settings to accomplish different objectives.

  • It can be used in paternity cases or criminal cases to determine the source of a DNA sample. (i.e. it has forensic applications).
  • It can be used determine the disease status of an individual. (e.g. it can be used in the detection of mutations particularly known muations)
  • It can be used to measure recombination rates which can lead to a genetic map with the distance between RFLP loci measured in centiMorgans.

Gel Image of RFLP



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