What is UPLC (Ultra Performance Liquid Chromatography)?

UPLC is an advanced liquid chromatography technique that utilizes columns packed with particles less than 2 µm in diameter. This allows operation at much higher pressures than traditional HPLC, resulting in:

  • 3–10× faster analysis time
  • 2–5× higher resolution and peak capacity
  • Improved sensitivity and reduced solvent consumption
  • Better separation of complex mixtures and closely related compounds

Our UPLC Capabilities & Instrumentation

Advanced UPLC Systems

  • Agilent 1290 Infinity II UPLC System
  • Waters Acquity UPLC H-Class & I-Class Systems
  • Binary and Quaternary solvent delivery systems with high-pressure capability
  • Photodiode Array (PDA), UV-Vis, Fluorescence, and Refractive Index detectors
  • Sub-2 µm particle columns (BEH, CSH, HSS technology)
  • Auto-samplers with temperature control and low dispersion

Key UPLC Services We Offer

  • Pharmaceutical Assay & Content Uniformity Testing
  • Impurity Profiling & Related Substances Analysis – Process impurities, degradation products, and genotoxic impurities
  • Stability-Indicating Method Development & Validation – Forced degradation studies (acid, alkali, oxidative, thermal, photolytic) per ICH Q1A–Q1E
  • Full Analytical Method Development & Validation as per ICH Q2(R1), USP <1225>, and FDA/EMA guidelines
  • Dissolution Testing for immediate and modified-release formulations
  • Bioanalytical Method Development for pharmacokinetic studies
  • Herbal & Nutraceutical Marker Quantification
  • Cleaning Validation & Residual Analysis

UPLC vs HPLC – Technical Comparison

ParameterHPLCUPLC
Particle Size3–5 µm<2 µm (typically 1.7 µm)
Operating PressureUp to 6000 psiUp to 15000 psi
Analysis Time15–60 minutes2–10 minutes
ResolutionGoodExcellent (2–5× higher peak capacity)
Solvent ConsumptionHigherSignificantly lower (up to 80% reduction)
SensitivityStandard3–5× higher

Our UPLC Workflow

  1. Project Consultation – Understanding regulatory requirements, analyte properties, and matrix challenges
  2. Sample Preparation – Optimized extraction and clean-up procedures for complex matrices
  3. Method Development – Systematic optimization of column chemistry, mobile phase pH, gradient profile, flow rate, and detection wavelength
  4. Method Validation – Comprehensive validation covering specificity, linearity, accuracy, precision, LOD/LOQ, robustness, and system suitability as per ICH Q2(R1)
  5. Routine Analysis & Reporting – High-throughput analysis with detailed chromatograms, calculations, and regulatory-compliant reports

Applications of UPLC Analysis

  • Pharmaceutical development and quality control
  • Stability and forced degradation studies
  • Bioequivalence and pharmacokinetic support
  • Herbal medicine standardization and adulteration testing
  • Impurity characterization and genotoxic impurity analysis

Why Choose Yaazh Xenomics for UPLC Services?

  • State-of-the-art UPLC systems with sub-2 µm column technology
  • Experienced team skilled in challenging method development
  • Full regulatory compliance documentation (ICH, USP, FDA, EMA)
  • Fast turnaround time for both development and routine testing
  • Cost-effective solutions without compromising quality
  • Seamless integration with LC-MS for unknown identification

Extending Our Capacities With UPLC

Putting any advanced technology to good use is the USP of our genomics services in South India. We are effectively armed with the finest DNA sequencing labs that enable us to deliver mission-critical, scalable solutions with UPLC. We deliver:

01
Qualitative analysis for the identification of individual compounds.
02
Quantitative analysis to measure trace amounts of compounds.
03
Quantitation and analysis of polymer additives.
04
Mapping of glycopeptides and peptides.
05
Testing of impurities and surfactant analysis.

Request UPLC Analysis Quote

Yaazh Xenomics Pvt Ltd
Module No. 103, TICEL BIOPARK Phase – III,
Maruthamalai Road, Coimbatore – 641046, Tamil Nadu, India

Phone: +91 99431 32020 | +91 95002 45454
Email: info@yaazhxenomics.com

Get A Quote
Yaazh Xenomics,
Module No. 103,
TICEL BIOPARK Phase – III,
1st floor, Maruthamalai Road,
Coimbatore - 641046.
Tamil Nadu, India
Yaazh Xenomics,
No.9-6, Sritej Nagar,
Anandapuram,
Visakhapatnam -531022.
Andhra Pradesh
Yaazh Xenomics,
Ground Floor, Plot No.17-R1,
120 feet Road,
Vivekananda Nagar,
Sambakulam, Madurai - 625 007,
Tamil Nadu. Indi

Yaazh Xenomics is a leading biotechnology company based in Coimbatore, Tamil Nadu, India, specializing in comprehensive genomic solutions. As a DNA testing laboratory, we offer a broad spectrum of services, including DNA sequencing, RNA Sequencing, Sanger Sequencing, 16s rRNA, 18s rRNA, ITS, COI, RBCL, Matk gene Sequencing for DNA Barcoding, gene expression analysis, SNP analysis, Next-Generation Sequencing (NGS), Various Medical Genome testing, Exome Sequencing, Gut Microbiome Test, Metagenome Sequencing, Whole Genome Sequencing (WGS), Transcriptome Sequencing using advance NGS platforms like Nanopore, Illumina, MGI, Thermo. Also, we provide advance Bioinformatics, Customized Bioinformatics and a variety of other genetic testing and Molecular testing.

 

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