Library prep, basecalling and hybrid polishing — and when each read length earns its place.
Oxford Nanopore has made closed bacterial genomes routine in any well-run lab. The difference between a fragmented draft and a finished genome usually comes down to library prep and polishing discipline — and to choosing the right read length for the job.
Protect read length at prep
Long reads only help if the DNA is long. Gentle extraction and careful handling preserve the high-molecular-weight fragments that span repeats and close the chromosome.
Basecall, then polish
Modern basecalling models have closed much of the historic accuracy gap, and a short-read polishing pass removes the residual systematic errors — yielding a reference-grade consensus.
Long reads for structure, short reads for accuracy — the hybrid finish is hard to beat.
- Gentle, high-molecular-weight extraction
- Current basecalling models
- Hybrid polishing for consensus accuracy
- Plasmid recovery confirmed by coverage
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SureshYaazh Xenomics
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