Technical Genomics FAQ Hub

Yaazh Xenomics is Coimbatore's leading affordable genomics and data sequencing lab specializing in NGS, Sanger, Nanopore, DNA barcoding, bioinformatics, and analytical testing for researchers, clinicians, and industries across India.

Main lab: Module No. 103, TICEL BIOPARK Phase-III, 1st Floor, Maruthamalai Road, Coimbatore - 641046, Tamil Nadu, India.

We provide some of the fastest TAT in India: Sanger sequencing (1-3 days), standard NGS projects 7-14 days (depending on sample volume and complexity).

Our standard long-reading sequencing process achieves a quality score of Q20 or higher (99% accuracy), with typical contiguous read lengths ranging from 800 to 1000 base pairs (bp), depending on template quality and primer specificity.

We utilize specialized sequencing chemistries, including the addition of DMSO, betaine, and high-temperature denaturing protocols (up to 98°C) to successfully sequence challenging GC-rich regions (>70% GC) that typically cause polymerase slippage or early termination.

Using a strategic primer walking approach with 500-700 bp overlaps, we can sequence constructs, plasmids, and BACs up to 10kb in length on both strands to ensure consensus accuracy.

Primers should ideally be 18-24 nucleotides long, with a GC content of 40-60%, and a melting temperature (Tm) between 55°C and 60°C. Please ensure your primer binding site is at least 40-50 bp away from your region of interest to account for the initial loss of resolution in the chromatogram.

Yes - full primer walking and custom sequencing for long amplicons or unknown regions. We design primers and extend coverage step-by-step.

We use Illumina (NovaSeq, NextSeq, MiSeq), MGI, Thermo, and Oxford Nanopore platforms for high-accuracy whole genome, exome, transcriptome, and metagenome sequencing.

We offer Whole Genome, Exome, Transcriptome (RNA-Seq), Metagenome (16S/Shotgun), Targeted Panels, ChIP-Seq, Methylation - and more for human, plant, animal, microbial samples.

Standard projects 14-30 days. We accept bio-fluids, FFPE, degraded samples. Minimum DNA/RNA quantities vary (e.g., 1-10 ng for most libraries). Contact us for project-specific requirements.

For clinical applications, we recommend a minimum coverage depth of 100x for Whole Exome Sequencing (WES) to ensure accurate variant calling in coding regions. For Whole Genome Sequencing (WGS), a depth of 30x is the industry gold standard for detecting structural variants and single nucleotide polymorphisms (SNPs).

16S rRNA sequencing targets specific hypervariable regions (e.g., V3-V4) to identify and quantify bacterial taxa. Shotgun metagenomics indiscriminately sequences all genomic DNA in a sample, providing not just taxonomic classification down to the strain level, but also functional pathway profiling across bacteria, viruses, and fungi.

For whole transcriptome analysis, we utilize magnetic bead-based ribodepletion kits (such as Ribo-Zero Plus) to efficiently remove cytoplasmic, mitochondrial, and chloroplast ribosomal RNA prior to library preparation. This maximizes sequencing real estate for informative mRNA and lncRNA transcripts.

Yes - free standard pipelines plus advanced customized data (variant calling, taxonomy classification, visualization, etc.) are included in most projects.

Standard deliverables include raw data (FASTQ), alignment files (BAM/CRAM), and variant call files (VCF). We provide customized pipelines including Differential Gene Expression (DGE), Gene Ontology (GO) enrichment, and comprehensive HTML reports with PCA plots and heatmaps.

Yes - secure high-performance servers for NGS, metagenome, transcriptome data with fast delivery of results.

Yes - clinical exome, cancer panels, rare disease diagnosis, pharmacogenomics with variant annotation and reporting.

Yes, we provide both pan-cancer and specific somatic mutation panels (e.g., BRCA1/2, EGFR, KRAS) with ultra-high sequencing depth (>500x to >1000x). This allows for the confident detection of low-frequency variants and subclonal mutations in heterogeneous tumor tissue, including FFPE samples.

Yes, all clinical variant curation and interpretations are strictly performed in accordance with the American College of Medical Genetics and Genomics (ACMG) and the Association for Molecular Pathology (AMP) framework.

We follow the latest ACMG recommendations for reporting secondary findings in highly penetrant, medically actionable genes (e.g., familial hypercholesterolemia, Lynch syndrome), provided the patient has not explicitly opted out during the pre-test informed consent process.

FFPE blocks and unstained slides should be shipped at ambient temperature. Ensure slides are securely packed in specialized slide mailers to prevent mechanical breakage during transit, and protect blocks from prolonged exposure to extreme heat.

Extracted RNA is highly susceptible to RNase degradation. Purified RNA samples must be shipped on dry ice to maintain a strict temperature of -80°C. Alternatively, tissues can be shipped submerged in RNAlater® stabilization solution at room temperature.

Extracted nucleic acids (DNA/RNA) are routinely stored in our -80°C biorepository for 90 days post-reporting. If you require extended biobanking services for longitudinal studies, this can be arranged under a separate service level agreement.