Ultra-Long Read Nanopore Sequencing
Full-length 16S / WGS / Direct RNA Sequencing. Uncover structural variants, complex repeats, and access native base modifications.
Workflow / Steps
Basecalling
Guppy/Dorado GPU basecalling for high Q-scores and native modification calling.
Assembly
Flye, Canu, Miniasm, and Medaka for high-accuracy long-read polishing.
Advanced Analysis
Structural variant calling (Sniffles), isoform detection, methylation profiling & AMR.
Deliverables
Publication-ready graphics, fully annotated genomes, FASTQ, BAM, and interactive reports.
Ultra-Long Reads
Resolve assembly of full complex genomes, map structural variations, and uncover sequences hidden in short-read data.
Real-Time Analysis
Rapid identification of AMR (Antibiotic Resistance) genes, pathogen detection, and fast environmental screening.
Native Sequencing
Sequence native DNA & RNA without PCR amplification, keeping base modifications intact (e.g. Methylation).
Our Nanopore Platforms
MinION Mk1C
Portable & Rapid Portable, rapid turnaround for small genomes and amplicon sequencing.
GridION
High-Throughput Benchtop Ideal for bacterial genomes, RNA-Seq, and small eukaryotes (up to 5 flow cells).
PromethION 2 (P2I)
Maximum Yield High-capacity automation for large plant/human genomes and complex cancer genetics.
Comprehensive Nanopore Solutions
Full-length 16S rRNA Metagenomics (V1-V9)
Higher resolution than traditional short-read V3-V4 for precise species-level identification.
Direct RNA Sequencing & Long-Read Transcriptomics
Sequence full-length RNA isoforms, alternative splicing events, and RNA base modifications without cDNA bias. • Detect alternative splicing • Identify novel isoforms • Map RNA modifications directly
Long-Read Whole Genome Sequencing
De novo assembly, gap closing, and T2T (Telomere-to-Telomere) assembly of complex genomes.
Plasmid / Virus / Phage Sequencing
Rapid, full-length circular assembly for vector verification and virology research.
Nanopore Long-Read vs Traditional Short-Read
| Feature | Nanopore Long-Read | Short-Read (Illumina) | |
|---|---|---|---|
| Read Length | Up to 4 Mb+ | 150-300 bp | |
| Structural Variants (SVs) & Repeats | Excellent | Limited | |
| Epigenetics (Methylation) | Native (Direct DNA/RNA) | Requires Bisulfite Conversion | |
| De novo Assembly Contiguity | Highly contiguous (T2T) | Fragmented | |
| Real-time Analysis | Yes | No |