Metagenome Analysis
Unlock the complex world of microbial communities. Utilizing 16S/18S/ITS and Shotgun Metagenomics, we provide end-to-end bioinformatics answering 'Who is there?' and 'What are they doing?'.
Publication Support Included
Full metadata analysis integration, robust statistical validation (PERMANOVA/ANOSIM), methodology write-ups, and figure generation tailored for high-impact journals like Microbiome or ISME Journal.
Tech & Pipelines
Instrument Compatibility
Amplicon Platforms: Illumina MiSeq (V2/V3), NextSeq 550, Thermo Fisher Ion S5, GeneMind GenoLab M. Shotgun Metagenomics: Illumina NovaSeq X, MGI T7, Oxford Nanopore (GridION/PromethION) for real-time surveillance.
Data Quality & AI Pipeline
• 16S Data: 100k - 500k reads/sample, Q30 > 75% • Shotgun Data: 5-10GB Raw Data/sample, Q30 > 80% • AI Utilization: We apply Kraken2 ML-based algorithms for rapid taxonomic classification and MetaBat2 for predictive functional binning of MAGs. Tools: QIIME2, DADA2, HUMAnN3, MEGAHIT, MetaPhlAn3, SILVA, KEGG.
Service Tiers & Deliverables
Amplicon Profiling
₹3,000 - ₹5,000 16S / 18S / ITS Diversity Analysis TAT: 10 - 15 Days Deliverables: OTU/ASV Tables, Krona Charts, Alpha/Beta Diversity Plots, LEfSe Biomarker Discovery.
Shotgun Functional Analysis
₹6,000 - ₹10,000 Comprehensive Strain & Pathway Mapping TAT: 3 - 4 Weeks Deliverables: Deep Taxonomic Profiles, Functional Gene Counts (SEED/KEGG), AMR (CARD) & Virulence Factor Profiles.
Analysis Capabilities
- 1. Taxonomic Diversity Profiling Amplicon or Shotgun data classification (Phylum to Species). • Alpha Diversity: Chao1, Shannon, Simpson (Richness & Evenness). • Beta Diversity: PCoA, NMDS (Bray-Curtis, UniFrac).
- 2. Functional Pathway Analysis Shotgun data mapped to reconstruct active biochemical pathways (Nitrogen, Carbon cycling, etc.) within the community.
- 3. AMR & Enzyme Discovery Targeted screening against the Comprehensive Antibiotic Resistance Database (CARD) and mining for industrial biocatalysts.
Study Environments
Clinical Microbiome: Gut, skin, and oral microbiome profiling in relation to disease states (Dysbiosis).
Environmental: Soil, ocean, and extreme environment metagenomics for ecological monitoring.
Industrial/Agriculture: Bioremediation tracking, fermenter community dynamics, and plant rhizobiome analysis.