Real-Time PCR (RT-PCR / qPCR) Services
Precise Gene Expression & Nucleic Acid Quantification
What is Real-Time PCR?
Real-Time Polymerase Chain Reaction (also known as quantitative PCR or qPCR) is a highly sensitive and specific technique that monitors the amplification of DNA or cDNA in real time using fluorescent reporters. Unlike conventional PCR, qPCR allows precise quantification of starting template amounts during the exponential phase, making it the gold standard for gene expression analysis, viral load determination, and pathogen detection.
Reverse Transcription qPCR (RT-qPCR) additionally converts RNA to cDNA, enabling quantification of mRNA, miRNA, and other RNA molecules.
Our Capabilities
Relative and Absolute Quantification
Gene Expression Analysis (mRNA and miRNA)
Transcript Variant Detection and Isoform Analysis
Viral Load Quantification and Pathogen Detection
Allelic Discrimination / SNP Genotyping
miRNA Expression Profiling
Validation of NGS / Microarray Data
High-Throughput Screening of Bulk Samples
Custom Assay Design and Optimization
Workflow / Steps
End-to-End RT-PCR Workflow
High-quality RNA / DNA extraction from various sample types (tissues, blood, cells, microbes, plants, FFPE)
Step 2
RNA quality assessment (RIN / integrity check) and reverse transcription (when required)
Step 3
Primer / probe design, validation, and optimization
Step 4
Real-time PCR setup with appropriate controls (no-template, no-RT, positive controls)
Step 5
Data acquisition and analysis using advanced software (Ct value, standard curves, efficiency, ΔΔCt method)
Step 6
Comprehensive reporting with graphs, statistics, and interpretation support
Ready for Accurate RT-PCR Results?
Let our experts handle your real-time PCR assays with precision and reliability. Save time and resources with trusted, high-accuracy Real-Time PCR services from Yaazh Xenomics. **Your reliable partner for gene expression analysis and molecular diagnostics**
Platforms & Chemistry We Use
TaqMan Probe-Based Assays
High specificity and multiplexing capability for precise quantification
SYBR Green Chemistry
Cost-effective option for gene expression studies with melt curve analysis
Agilent & Affymetrix Platforms
Advanced real-time PCR systems for high-throughput applications
In-house Synthesis
In-house primer and probe synthesis for faster turnaround and optimized performance.
Key Applications
Our RT-PCR service is a powerful tool for a broad spectrum of applications from fundamental research to applied biological research.