As part of our genomics services, Yaazh offers fast, precise primer walking services. We identify both PCR fragments or entire plasmids. Going a step beyond, we analyse base sequences that cannot be identified with single primer extensions.
First, we sequence the bases of the PCR product or plasmid provided to us by the customers. We do either one side end sequencing or both. Then, we design and prepare internal primers using the data obtained. These primers then ‘walk’ further down the sequence. We offer continuous extensions for plasmid DNA or PCR produce that cannot be read by single primer extension sequencing.
With this service, we sequence a single strand of the entire fragment or product. We guarantee greater than 99.9% final data accuracy.
With this service, we sequence both strands of the fragment or entire product. We further design primers if the resulting sequence is ambiguous. Yaazh ensures 99.999% accuracy with < 1 error in 1,00,000 base pairs.